The second edition of the ASEAN Biodiversity Outlook provides an assessment of the implementation of the Strategic Plan for Biodiversity 2011–2020 and the Aichi Biodiversity Targets in the ASEAN region. Through a “Threats, Response, and Way Forward” framework, it discusses the state of major habitats found in the terrestrial and aquatic realms of the ASEAN Member States, how these are affected by drivers of biodiversity loss, and how the AMS have stepped up to address such issues and articulated ways forward to meet the Aichi Biodiversity Targets.